palb2 c ter  (Bioss)

Journal: Biomedicines

Article Title: Functional Disruption of IQGAP1 by Truncated PALB2 in Two Cases of Breast Cancer: Implications for Proliferation and Invasion

doi: 10.3390/biomedicines13081804

Figure Lengend Snippet: Key domains and interaction regions of PALB2 protein and the P-T1 and P-T2 variants. ( A ) Domains and interaction regions of the PALB2 protein. The upper circles show the molecular surface representation of the Coiled–coil PALB2 (orange) dimerization, coil–coil PALB2-BRCA1 (green) interaction, PALB2-MRG15 (yellow) interaction, and PALB2-BRCA2 (cyan) interaction. The lower circles show the intrinsically disordered structure of the N-terminal (AlphaFold 2 prediction) and the β-sheet structure of the WD40 domain at the C-terminal. Ab1 and Ab2 represent the antigenic regions recognized by the PA5-48258 and BS-0588R PALB2 antibodies, respectively. ( B , C ) Diagram of the domains and interaction regions of PALB2 variants P-T1 and P-T2, respectively. CC, coil–coil motif; ChAM, chromatin association motif; FX, FXLP motif; NLS, nuclear export signal; BRCA1, BRCA1 DNA repair-associated; KEAP1, Kelch-like ECH-associated protein 1; RAD51, RAD51 recombinase; MRG15, MORF-related gene 15; BRCA2, BRCA2 DNA repair-associated; RAD51C, RAD51 paralog C; XRCC3, X-ray repair cross complementing 3; POLE, DNA polymerase epsilon (catalytic subunit) and RNF168, ring finger protein 168.

Article Snippet: PALB2 (C-ter) , , Rabbit polyclonal , , 1:200 , Bioss 2 , BS-0588R.

Techniques:

Journal: Biomedicines

Article Title: Functional Disruption of IQGAP1 by Truncated PALB2 in Two Cases of Breast Cancer: Implications for Proliferation and Invasion

doi: 10.3390/biomedicines13081804

Figure Lengend Snippet: PALB2 full-length protein. Western blot of isolated proteins from HeLa cells and from circulating white cells from control IDC and cases with truncated variants of PALB2, P-T1, and P-T2 probed with PALB2 C-terminus antibody.

Article Snippet: PALB2 (C-ter) , , Rabbit polyclonal , , 1:200 , Bioss 2 , BS-0588R.

Techniques: Western Blot, Isolation, Control

Journal: Biomedicines

Article Title: Functional Disruption of IQGAP1 by Truncated PALB2 in Two Cases of Breast Cancer: Implications for Proliferation and Invasion

doi: 10.3390/biomedicines13081804

Figure Lengend Snippet: PALB2 (green) and IQGAP1 (red) expression in breast cancer control (CC1 and in CC2) mutated breast cancer (P-T1) labeled with antibodies binding to N-terminal or C-terminal domains. In CC2, PALB2-specific fluorescence at high intensity in cytosol and to a lesser extent in the nuclei of tumor cells (white arrowheads); IQGAP1-specific immunofluorescence in the plasma membrane and cytosol of most cells (yellow arrows) at two different intensities, high and medium level, in a dot-like pattern distribution; in the PALB2-IQGAP1 merged image, these two levels are more evident, and colocalization exists to a certain extent. B-T1 anti-PALB2-N-terminus antibody immunofluorescence signal present in both cytosol (high) and nucleus (medium and homogeneous); IQGAP1-specific immunofluorescence is displayed at a homogeneous medium intensity level in the plasma and cytosol of most cells. C-terminus anti-PALB2 antibody in CC1 samples exhibited similar immunolabeling to the N-terminus (white arrowheads). The IQGAP1 localization pattern of two intensity levels and localization is also shown in the first lane of the images. B-T1 and C-terminus anti-PALB2 antibody fluorescence at medium–high intensity, mainly in cytosol and at much lower intensity in the nuclei of cancer cells (white arrowheads). IQGAP-specific immunofluorescence at medium- to high-level intensity in cytosol and plasma membrane, frequently colocalizing (merged) in some tumor areas (yellow arrows). Scale bar = 40 µm.

Article Snippet: PALB2 (C-ter) , , Rabbit polyclonal , , 1:200 , Bioss 2 , BS-0588R.

Techniques: Expressing, Control, Labeling, Binding Assay, Fluorescence, Immunofluorescence, Clinical Proteomics, Membrane, Immunolabeling

Journal: Biomedicines

Article Title: Functional Disruption of IQGAP1 by Truncated PALB2 in Two Cases of Breast Cancer: Implications for Proliferation and Invasion

doi: 10.3390/biomedicines13081804

Figure Lengend Snippet: Diagrams of PALB2 and IQGAP1 expression in truncated PALB2 human breast invasive ductal carcinoma. AU—arbitrary units. ( A ) Specific PALB2 expression in nucleus or cytosol in control (CC) IDC and in truncated PALB2 (P-T) IDC samples probed with antibodies against N- or C-terminus of PALB2 protein (* p < 0.05). ( B ) IQGAP1 total expression and peri-plasma membrane expression ( p < 0.05). ( C ) In subpopulations of cells (25–30% of total cells), correlation between the expression of IQGAP1 and PCNA evidenced that high expression of PCNA corresponded to lower expression of IQGAP1, which was higher in truncated PALB2 cases ( p < 0.05). ( D ) Correlation of IQGAP1/CK7 expression-level quotients in control and in PALB2-truncated cases. The correlation is lower in PALB2-truncated cases ( p > 0.05). In ( C , D ), point clouds are not represented to improve the clarity of the plots. ( E ) The Pearson correlation coefficient (PCC) was calculated for each sample to quantify the degree of colocalization between fluorophores.

Article Snippet: PALB2 (C-ter) , , Rabbit polyclonal , , 1:200 , Bioss 2 , BS-0588R.

Techniques: Expressing, Control, Clinical Proteomics, Membrane